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RP-HPLC Method Development for Lamivudine Estimation

M.Pharm Dissertation: Validation in Bulk and Tablet Dosage Forms

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RP-HPLC Method Development for Lamivudine Estimation
Dissertation submitted to Savitribai Phule Pune University | M.Pharm (Pharmaceutics) 2025–2026
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RP-HPLC Method Development for Lamivudine Estimation
Dissertation submitted to Savitribai Phule Pune University | M.Pharm (Pharmaceutics) 2025–2026
RP-HPLC Method Development for Lamivudine Estimation1 / 11
Contents
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  • ●
    Introduction to Pharmaceutical Analysis & Chromatography
  • ●
    HPLC: Principles and Components
  • ●
    Aim and Objectives
  • ●
    Plan of Work
  • ●
    Result and Discussion
  • ●
    Summary, Conclusion, and Future Scope
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Introduction I
Pharmaceutical Analysis & Chromatography
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Pharmaceutical Analysis Overview
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  • ●
    Identification and quantification of substances
  • ●
    Qualitative analysis: confirms identity
  • ●
    Quantitative analysis: measures purity/concentration
  • ●
    Chemical methods: titrimetric, gravimetric, volumetric
  • ●
    Instrumental methods: spectroscopic, chromatographic, electrochemical
  • ●
    New methods needed for non-pharmacopoeial drugs or interference issues
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Chromatography Fundamentals
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  • ●
    Separation technique using stationary and mobile phases
  • ●
    Based on differential affinity of compounds
  • ●
    Origin: Mikhail Tswett (1903) separated plant pigments
  • ●
    Term derived from Greek: chroma (color) + graphein (to write)
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Tswett's Early Chromatography
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Diagram of Tswett's calcium carbonate column separating plant pigments
Diagram of Tswett's calcium carbonate column separating plant pigments
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Introduction II
High Performance Liquid Chromatography (HPLC)
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HPLC Evolution and Types
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    Evolved from low-pressure liquid chromatography
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    Jim Waters (1970s): introduced high-pressure pumps
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    Types: Normal Phase, Reverse Phase, Size-Exclusion, Ion-Exchange
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HPLC System Components
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Schematic of HPLC system: Pump, Injector, Column, Detector, Recorder, Degasser, Column Heater
Schematic of HPLC system: Pump, Injector, Column, Detector, Recorder, Degasser, Column Heater
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HPLC Applications
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  • ●
    Pharmaceutical quality control
  • ●
    Environmental monitoring
  • ●
    Forensic analysis
  • ●
    Food and flavor analysis
  • ●
    Clinical testing
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Advancing Analytical Methods for Lamivudine
Questions & discussion, contact email or link...

RP-HPLC offers precision for Lamivudine estimation • Method development addresses gaps in existing techniques • Validation ensures reliability for bulk and tablet dosage forms

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